The Daily Dongle

FlowJo 7 and CSV export

This question just came up on PUCL, so I probably should mention the few ways of handling CSV data in FlowJo 7. This pretty much applies for the version 9 as well. The simplest use case is you have a bunch of FCS files and want to convert all of... Read more →

vertex editing rant

semi-tutorial, semi-rant - this is to address removal of vertices from a polygon gate, and resulting performance differences, in FlowJo mac/java. Spoiler alert: fewer vertices = faster. Download Vertex tutorial [MOV, 6.4MB] Read more →

Autobatching & Command Line tutorial

Download autobatch tutorial [MOV, 18.2MB] Summary: Auto-Batching is a feature which can be utilized when running flowjo in Command-Line Mode (CLI). CLI was developed to give the set of features necessitated by large volumes of data set that may need automated pre-processing prior to 'attended' analysis - pre-gating, compensation calculations,... Read more →

performance issues

FlowJo V7 (Java) performance is an ongoing issue in our support and PUCL. This post is a Cliffnotes of existing DD posts on the subject. Following these steps usually resolves 3/4 of our users' gripes on performance, while the remaining quarter use gigantic (hundreds MB) files and deeply nested (10+... Read more →

converting keyword $Vol into absolute cell counts in gates (Accuri C6 data)

This is a tutorial which revolves around the keyword $Vol found in Accuri data. Someone asked, how they can use it in FlowJo to convert gate stats into absolute counts of cells. The background on this is that $Vol is a keyword found in some Accuri data which contains the... Read more →

comp-matrix confetti? badges to the rescue

You might have noticed that starting with version 7.6 there are new ornaments in the workspace window, to the left of the sample names. We call them "badges" : each of the three has a different context, for now, I'd like to focus on the right-most - which represents compensation.... Read more →

FlowJo Limits

Question: I want to know what FlowJo's limits are - i.e. how many files with how many events can be stored in a workspace. Most files in Workspace most I ever tired was 3000 FCS files. Not because it crashes at 3001st but because that's all I had at the... Read more →

Population Comparison tutorial

Download Population video tutorial, 15MB (quicktime) This is to present a slight twist on the interface as you may have seen it in the Mac version. Read more →

data range adjustment video tutorial/rant

Dataranges (quicktime, 13MB) this is to introduce the new tool for adjusting range parameters in high-res FCS3 data (Accuri, XDP, etc.) This is the first implementation, we expect to improve it for flowjo x, given your feedback. This has been in all of the betas from the past 2-3 months... Read more →

64 bit flowjo, XDP data

news on the 7.6 front: we successfully opened a 2.18GB file generated by DIVA in the 7.6 version of FJ, compiled in 64 bit already, since we made it available for the mac. I just never got a file big enough to test that it's really 64 bitty. :) The... Read more →