The Daily Dongle

Copy Keyword values from Excel to Flowjo X (Mac)

Hello FlowJo Gurus, Lately, we have gotten a few requests in FlowJo tech support from FlowJo X v10.0.7 users on a Mac trying to copy/paste keyword values from an Excel spreadsheet into a FlowJo workspace. Unfortunately, Excel for Mac uses the ANSI character set and FlowJo X wants Unicode characters.... Read more →

Script Editor Keyword and Index Sorting Example

The FlowJo X Script Editor allows the user to automate and extend the features in FlowJo (Java version only). When creating FlowJo scripts, it will be helpful to know some javascript, here is a reference tutorial, and here is the FlowJo X Script Editor manual page. Sample Sorting by Collection... Read more →

FlowJo Calibration to convert Intensity into Concentration

A few questions have come in lately to FlowJo tech support (flowjo@treestar.com) asking about Cytometric Bead Array (CBA) analysis in FlowJo. FlowJo does not have a special platform for CBA yet, but it is fairly quick to make the analysis with the Table Editor, and Derived Parameter platforms, which allow... Read more →

Ribbons PSA

As new users switch from V9 to Vx, we continue to get the occasional "grumbles, ribbons!" email. Here are some quick and easy ways to get those pixles off your screen, unless you need them. Notice the area circled in red in the screenshot above. if you double click it..... Read more →

Cytometer Prefs and Data Scaling

In FlowJo Vx, by default, we will scale data parameters based on the keywords in your data. In some use cases, FlowJo users wish to alter these scales. We introduced storing these in preferences in V7, but it was done in a global way, meaning if you "tuned" your settings... Read more →

Speed tricks for analyzing Large Data Files

Some flow researchers with large event count files, ask us for tips to speed up the FlowJo analysis. Here are a couple quickies. Preprocessing If the analysis workflow allows it, one can do some pre-processing. In FlowJo, gate on Cells of interest (eliminating debris and doublets), then use the Export... Read more →

OSX 10.8 and zip file blues

if you are on one of them fanshy upgraded OSX 10.8 computers and hit an error about a "corrupted file" when accessing one of our downloads in Zip format, apparently you have to go to Security/Privacy OSX preference panel and click as arrowed below: This will enable your ability to... Read more →

new tricks for site-wide installations

starting with the version mentioned in this post, and any builds that follow, I've put in a convenience for site wide installations of FJ. FlowJo will look at the environment variables: FJSERIALNUMBER FJPROXYPORT FJPROXYHOST and if they have usable values, use them accordingly. This way if you can set these... Read more →

Astrios (and MoFlo XDP ) data

in lieu of mixing this long winded rant with today's beta fixes, I'm putting it in this post. To achieve better scaling for Astrios and MoFlo data, we had to mess with preferences, and Engine code. A full preference reset is recommended before this beta is used, specifically ensuring that... Read more →

FlowJo v765 Mac Command Line

FlowJo v7.6.5 has a nifty command line feature extension for templates. (The FlowJo license allows Mac users to run both our C version (currently v9), and our Java version (currently v7.6.5); the feature sets of the 2 programs being slightly different). For repetitive analysis workspaces amenable to templates, the command... Read more →