The Daily Dongle

Cell Coloring Contest!

Cytometry is all about COLOR and we want to see your best take on a single cell! Just upload a photo of the cell to Instagram or Facebook decorated however you want (colored pencil, paint, collage, etc.) and tag @FlowJoLLC on Instagram and FlowJo, LLC on Facebook by Feb. 24th... Read more →

Free Webinars in February

Did you know that we host FREE webinar tutorials every month? Every first and third Thursday you can get special training on FlowJo® from one of our experts. No advanced registration is required for these free classes! Make sure to add the webinar links below to your calendar so you... Read more →

Is FlowJo® Sluggish on your Machine? Check the Following

One of the most frequently asked questions we get from users is: “How can I optimize FlowJo’s performance?” The short answer is, FlowJo attempts to optimize settings automatically for your machine during installation. How convenient! The number one cause of sluggish performance in FlowJo comes down to RAM available on... Read more →

Email Notification Plugin

The Email Notification plugin promotes communication by sending automatic email notifications when a FlowJo® workspace is saved. In FlowJo Client, the user can modify the plugin settings. When the workspace is saved, the settings window appears containing sender information and a list of email notification recipients. The plugin settings are... Read more →

Free Webinars in January

Did you know that we host FREE webinar tutorials every month? Every first and third Thursday you can get special training on FlowJo® from one of our experts. Make sure to add the webinar links below to your calendar so you don't miss out on all the tips and tricks.... Read more →

Combining Samples—Concatenation with Added Keyword Parameters

By labeling samples with a numerical keyword that uniquely identifies the separate files, you can both: combine populations from different files into one FCS data file, and create a new parameter from any such keyword. Combining populations in FlowJo® is easy using concatenation. Simply select a set of populations you’d... Read more →

How to Use FlowJo’s Script Editor with Index Sorted Data

Index sorting is a method that deposits individual cells from a heterogenous mixture into wells of a plate (96, 384, and so on). Sorted cells are usually selected by some criteria (such as GFP+ and CD11b+, but CD3-) and then channeled into an empty well. Cells that do not meet... Read more →

Cell Ontology Plugin

The Cell Ontology plugin queries a cell ontology database and matches the cell or population in your FlowJoTM workspace to specific cell types. Almost all applications in flow cytometry rely on phenotype markers, and the CellOntology database maintains a record of published definitions of cell types with a myriad of... Read more →

Free Webinars in December

Did you know that we host FREE webinar tutorials every month? Every first and third Thursday you can get special training on FlowJoTM from one of our experts. No advanced registration is required for these free classes! Make sure to add the webinar links below to your calendar so you... Read more →

FlowJo Works with CytoFlex Data

By: John Quinn, PhD A fun perk of working as a FlowJo, LLC application scientist is getting a tour of the lab I’ve visited for training. As an admitted cytometry nerd, I enjoy getting to see new technology, to hear how a given acquisition machine or probe is working out,... Read more →