Upcoming Free Webinars

Did you know that we host FREE webinar tutorials every month? Make sure to add the webinar links below to your calendar so you don't miss out on all the tips and tricks.

 

Intro to FlowJo Envoy 
July 20th 2017 at 10:00AM PST

FlowJo Envoy is a cloud based lab organization & management software. It provides an individual lab or larger institution with the ability to house workflow instructions, progress reports, data storage, and workgroup specific communication in one place. This demo will show you how to simplify your lab organization in just moments. Click here to join. 

Concatenation and tSNE in FlowJo® 
August 17th, 2017 at 10:00 AM PST

tSNE is a dimensionality reduction tool designed for assisting in the analysis of data sets with large numbers of parameters. tSNE produces two new parameters that effectively summarize all of the information contained in all other parameters. To compare different samples using tSNE, you must first concatenated (merge) the individual samples you wish to compare, then run tSNE on the concatenated file to produce a common dimensionally reduced data space. You can then use gating to pull apart the concatenated file into individual samples and compare how each of those samples is represented in the dimensionally reduced data space. To discover phenotypic changes or shifts between experimental conditions, gate the distinct populations you see in the common tSNE plot, use the original parameters to immunophenotype the populations and then compare the prevalence of those populations of between experimental conditions. Click here to join.

 

Compensation 
September 21st, 2017 at 10:00 AM PST

The foundation of any multicolor flow experiment is always proper compensation. This is typically the first step in an analysis - relying on quality single stained compensation controls, either from beads or cells. Learn to: gate positive and negative control populations, troubleshoot the compensation platform, add/remove parameters, and check the calculated spillover matrix. Compensation can be tricky for novice and advanced cytometrists alike, but with a little practice, FlowJo's compensation Wizard feature will make this process a breeze. Click here to join.

 

 

Comments

[Martin]

Thanks