The Daily Dongle

Combining Samples—Concatenation with Added Keyword Parameters

By labeling samples with a numerical keyword that uniquely identifies the separate files, you can both: combine populations from different files into one FCS data file, and create a new parameter from any such keyword. Combining populations in FlowJo® is easy using concatenation. Simply select a set of populations you’d... Read more →

How to Use FlowJo’s Script Editor with Index Sorted Data

Index sorting is a method that deposits individual cells from a heterogenous mixture into wells of a plate (96, 384, and so on). Sorted cells are usually selected by some criteria (such as GFP+ and CD11b+, but CD3-) and then channeled into an empty well. Cells that do not meet... Read more →

Cell Ontology Plugin

The Cell Ontology plugin queries a cell ontology database and matches the cell or population in your FlowJoTM workspace to specific cell types. Almost all applications in flow cytometry rely on phenotype markers, and the CellOntology database maintains a record of published definitions of cell types with a myriad of... Read more →