FlowJo Calibration to convert Intensity into Concentration

    A few questions have come in lately to FlowJo tech support (flowjo@treestar.com) asking about Cytometric Bead Array (CBA) analysis in FlowJo. FlowJo does not have a special platform for CBA yet, but it is fairly quick to make the analysis with the Table Editor, and Derived Parameter platforms, which allow unit conversion from relative intensity to concentration using control samples.

The screen shot below from FlowJo X seems to help users with the work flow. 
(double click it to enlarge):

 

 

One can also concatenate the different standards, converting Keywords into parameters during concatenation in FlowJo v9, rendering plots like in the screen shot below:

 

 

(Note, the FlowJo X programmers are working on this concatenate-keyword-to-parameter feature, and FlowJo v9 (Mac-only) has a Calibration platform that does interpolation, but lacks the Table Editor Correlation plot feature.)

 

Comments

[Microhope]

Any chance version 10 (for Mac) has a CBA analysis feature yet? If not, how similar is the analysis flow through, compared to the one shown above for version 9? Might there be an updated post on the horizon (or does it exist and I didnt find it)?

Thanks for your help!

[Evaggelia Liaskou]

Hi,

can you please provide some more specific guidelines/instructions on how to do this analysis on either Flowjo9 or Flowjo 10?

That would be really useful.

Thanks.

[Flow Rida]

Seriously, can y'all come up with something for CBA?
jeesh.