The Daily Dongle

biexponential cramps

Every once in a while, Version 8 of FlowJo does a funny thing to some of the parameters when biexponential scaling is enabled. It looks something like... notice how the X parameter is squished from 0 to 10^4 above. FlowJo has two modes for calculating biexponential scaling - global (all... Read more →

Population Comparison

Your Mac version has a population comparison platform, which returns differences between my sample and control using four different algorithms. How can I compare them? Which one is better? This question demonstrates a need for better documentation of this Platform. The short answer is - there is no way to... Read more →

Powerpoint 2008 Mac - no love for PICT

FlowJo graphs/layouts are no longer ungroupable in the new version of 2008. If you find this functionality useful, stick to 2004 version. I found that 2004 version's ungrouped slides are still ungrouped in PPT 2008. Read more →

Randomization

From tech support: How do I process a random sampling of events form my file/gate? There is a way to randomize, but only in version 8 of the software. It involves exporting a certain number of events - either from the top-level node (ungated file) or one of it's subpopulations... Read more →

New Mac Pro's and FlowJo V8

With the new crop of Macs on Apple Store, I thought I'd revisit my hardware recommendations for an analysis workstation. Data Input: I saw no benefits going with expensive disks - if you can get the regular sata-2 disks @ 7200RPM, that's plenty. I did not see a significant increase... Read more →