The Daily Dongle

Negative Fluorescence

I saw an interesting post by Dr. Roederer on Purdue list archives discussing the topic of negative fluorescence. This question came up in my email as well just last week, so it's nice to get a real scientist's (as opposed to us flow-jocks) perspective: Recently, I was queried by a... Read more →

polyvariate plot tutorial

FlowJo for Macintosh includes a tool called "polyvariate display". This allows our users to define multidimensional populations with a single scatter plot and 2D gates. I'm presenting this as a quick time movie, 18 minutes long, 33MB. It's a new venture for the DD, perhaps all future entries will be... Read more →

Friday Webinar?

Would any of the 'Dongle readers be interested in a free Webinar? we have a WebEx account and can host interactive tutorials. I could think of a thing or two to demo.. How about Friday some time? Email me if you're interested (I'll need at least 5 people! ) flowo... Read more →

Undo on Mac FlowJo

Some months back we've implemented a general undo functionality in the Macintosh version of FlowJo. The testing so far suggests that it created some trouble for folks - crashing the program, corrupting workspaces, etc. And unfortunately you can't undo a bad undo ;) So.. please click FlowJo menu, Preferences, and... Read more →

the easiest and fastest way to do software compensation on FCS data

Starting in 8.4b3, the compensation wizard is fully automated. No gating is required. Here's a brief demo: 1. drag the compensation samples from the file system to a flowjo workspace (if you already have them in the workspace, make sure they're in their own group). This trick works best if... Read more →