The Daily Dongle

resetting window positions in FlowJo

FlowJo for windows saves widnow positions. If you change workstations, monitor configuration, or resolution, some windows in FlowJo may be painted wayyyyy off screen. The only way to reset window positions is to manually open the .wsp file in wordpad, and search for lines which begin with Read more →

Q&A - scales, channels, binning

I am using FlowJo to export .fcs files to .txt files. I noticed that there are two ways of exporting data: by channel and by scale. Which one should I use? channels correspond to values which have been scaled to the way you're used to look at your data. This... Read more →

Messing with DiVa compensation values in FlowJo for Macintosh

if you want to mess with DiVa compensation values in FlowJo, do it right. To do it right, follow this script: 1. add your diva files to a workspace. Add a column for the keyword "Compensation Matrix". Your workspace should look something like this. 2. Click on a sample in... Read more →

FlowJo for Windows manual in PDF

Harold writes: Dominic and I have developed a method to turn our online, web based, manuals into a PDF document with a Table of Contents, and page numbers. Please take a few minutes to check it out on our "internal web site" or at http://www.flowjo.com/FloWinMan.pdf If there are manual pages... Read more →

Mac version benchmarks, Intel and G5 across V4,6,8

http://www.flowjo.com/home/intelmac.html I've done some benchmarks (details in the link) and Tom created a nice graphical summary. Check it out. Read more →

herzenbergs on data vizualization/interpretation

There is a wonderful commentary from the Herzenberg laboratory in this month's Nature Immunology on interpreting/visualizing/analyzing FACS data... I highly recommend it to everyone! http://www.nature.com/ni/journal/v7/n7/index.html .... pp 681 - 685. also http://herzenberg.stanford.edu/Publications/Reprints/LAH516.pdf Read more →

DiVa log-shmog...

I get a lot of emails like I acquired my data in log, but your software is showing it in linear... This is usually wrt DiVa files. DiVa files are in FCS3.0 format (see this and that )and all parameters are linear. FlowJo has preferences which will try to scale... Read more →

How to roll your own FCS files

*updated 2/18/2010* - added a new section with demo file, video tutorial and some comments at the bottom * This recipe will allow you to convert non-FCS database tables into FCS files for use with FlowJo software. The data file MUST have EXACTLY the following format. Each line is delimited... Read more →