The Daily Dongle

Combining scRNA-seq and Flow Cytometry Analysis

Combining scRNA-seq and Flow Cytometry Analysis Researchers generating sequencing data after index sorting in flow have a powerful set of multi-omic datasets that can and should be used in combination for analysis. Fluorescence information from index sorted FCS data is easily exported using FlowJo's index sorting plugin. This data can... Read more →

FLS Server February 23, 2018

This morning around 10:30 am, we experienced a brief connectivity issue with our FlowJo Licensing Server, which has been resolved. For further questions or concerns please reach out to: [email protected] or [email protected] Apologies for this inconvenience, The FlowJo Team Read more →

Upcoming Free Webinars

Did you know that we host FREE webinar tutorials every month? Make sure to add the webinar links below to your calendar so you don't miss out on all the tips and tricks. Intro to FlowJo Envoy July 20th 2017 at 10:00AM PST FlowJo Envoy is a cloud based lab... Read more →

Trouble Installing FlowJo v10.3 on Windows?: The Fix is In!

First, a synopsis of the error: You’ve just installed FlowJo and are now trying to run the application. You double-click the icon, only to see the FlowJo splash screen flash across the monitor, disappear, and then…nothing. The application somehow fails to run. Some Windows 10 users undoubtedly have run into... Read more →

DragonHacks

At Team FlowJo, we’re excited to expand the depth and breadth of algorithms available to the flow community via the FlowJo Exchange, our plugin sharing site. The Exchange currently hosts roughly fifteen plugins or scripts, and is growing. However, most of these tools were developed internally. Too truly make this... Read more →

Upcoming Free Webinars

Did you know that we host FREE webinar tutorials every month? Make sure to add the webinar links below to your calendar so you don't miss out on all the tips and tricks. Cell Cycle in FlowJo Date: Thursday, April 20th, 2017 Time: 10 AM PST In this webinar we... Read more →

LIMS/Database Integrator Plugin

I’m Shaun Rasmusen, summer 2016 intern at FlowJo, LLC, and I worked on developing the LIMS/Database Integrator Plugin. The purpose of this plugin is to provide an easy way to log information to a database about a workspace, its users, and the open/save times of the workspace. The design of... Read more →

Demystifying Compensation in FlowJo®

The Compensation Wizard in FlowJo is one of the most frequently used platforms, and by extension potentially the greatest source of confusion on a per-cytometrist basis. This brief guide is to help users new to compensation calculations and experienced flow-maestros alike breeze through this process in a painless and professional... Read more →

Free Webinars in March

Did you know that we host FREE webinar tutorials every month? Every first and third Thursday you can get special training on FlowJo® from one of our experts. No advanced registration is required for these free classes! Make sure to add the webinar links below to your calendar so you... Read more →

Calculating the Number of Molecules on Cells Using FlowJo v10

Measuring the fluorescence intensity of cells and particles is routine and the basis of the vast majority of inquiry in flow cytometry. Considering that fluorescence intensity is correlated with molecules on the surface of the cell, can the relationship between the two be quantified? If so, how can we use... Read more →