Ever want to look at how a statistic varies Day-to-Day from Patient-to-Patient on a single plot as shown in the graph below? One strategy is to use FlowJo v9, combining the use of keyword formulas and concatenation.
The dataset below contains a T-cell panel for two patients with tubes run on different days. The tube files contain keywords for Patient and Day (Our Advanced Tutorial Dataset). Gates were made in the workspace to get the frequency of CD4+CD8+ T-cells.
To make the graph:
- Create a keyword from the Freq of CD4+CD8+ statistic.
(see http://flowjo.typepad.com/the_daily_dongle/2011/07/focus-group.html)
-Concatenate the files, creating additional parameters for the Day, Patient, and CD4+CD8+ keywords.
(see http://flowjo.typepad.com/the_daily_dongle/2008/06/how-the-new-concatenation-works.html)
-Load the concatenated file back into the workspace, and display CD4+CD8+ vs Day.
-Switch the default pseudo-color plot to Polychromatic, assigning a color to the Patient parameter.
Or, another way to visualize and gate on the data:
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