You can view a FlowJo graph or layout in movie form. It's quick and easy, it won't hurt the data, and you may be surprised at what you see.
To make a movie, you add an additional parameter to the graph then view the data in relation to 30 or so slices of that parameter. If time is already a parameter in your data it will be the default choice.
But don't get bogged down in mere scientific details. The point here is to just try it once and see if you like the perspective.
- Open a graph and set the axes to forward scatter and side scatter.
- Choose psuedocolor as the graph type and put a check in the Smoothing box.
- From the Graph menu select Make Movie. You will see the dialog below.
- Select a parameter to be your "control."
- Select the Display accumulates..." button to show the data accumulating as more channels of the control parameter are displayed in the movie frames. The Show movie... option is selected by default. You DO have the QuickTime player installed, don't you?
- Click on Make Movie choose a name and directory and wait a moment for the frames of your movie to be created and displayed.
Other graph types make equally interesting movies. You need not choose psuedocolor. The first check box toggles between showing an equal number of the control parameter's channels per frame and and showing an equal number of events per frame. Try both...
You can scrub through the movie by moving the "play head" triangle back and forth along the time line. You can also scrub the movie by clicking or pressing and holding the arrow keys on the keyboard.
You can view the movie I made as I wrote this here.
For all the movie-making details, you can read the Web docs.