July 03, 2009

new way to set number of decades on log plots

In an upcoming Mac 8.9 release, this dialog (FlowJo > Prefs > Workspace > Define..)  has new clicks:

Picture 11

Specifically in the middle section called "Standard Log Conversion Settings" :

Picture 10

The Setting-Formerly-Known-as-Starting Linear Channel Number is now simply called "lowest". This setting, in the units of channels, controls the left edge of the scale. If you type in 1000, you will chop the first 1000 channels from your data, and it will start at 10^3 instead of the usual 10^0.

The new setting, "highest", is in the units of decades. If you leave it at 0, the max value of the scale stays at what it was originally (for example, Accuri files go to 7.2 decades, diva - 5.4, etc.

Notice that the two settings are mutually exclusive.

The second control after you pick your left or right edge, is the number of decades in the "pulse area/height" text fields.

In the screenshot shown, I'm setting my prefs for Accuri data. I want to shave 0.3 off the top (assuming my fluorescence range usually doesn't hit the max value) and show what's left as 5 logs. Which would look like this:

Picture 13

Which looks more manageable than the full scale:

Picture 14

Look for the 8.9 beta, out soon.

July 02, 2009

FlowJo translation project

We're starting to translate FlowJo into other languages. Please leave a comment on this post if your language should be added to the list. Currently we are making progress in Chinese and Polish (we just happen to have prolific translators fluent in these, as well as German and French, which will likely be next.)


 Anything else?  

Picture 9

We might be interested in translators for languages other than the four mentioned - unicode experience is a must. Let us know if you'd be into it, and maybe we can discuss how to make it worth your while (probably limited to feel-good-points and T shirts.) FlowJo pratar inte Svenska!

June 26, 2009

FlowJo 7.5.3 and engine null problem

There is a sporadic problem affecting bout 7 users (to date) where FlowJo's engine does not load and notifies the user with that info, then either quits, or does not work right.

WE have been working on this one for two days now, with even less understanding of the problem than when we started :) we have made some progress, however, to improve our diagnostic tools to help us get more consistent info from your computer, which will in turn - help us fix the problem.

Please download and expand the zip file linked below, and follow the instructions in README.txt - contact flowjo@treestar.com if you have any questions or need help with this.

Download 7.5 diagnostic toolkit

June 23, 2009

Fran's guide to flowing in R

Fran wrote up an excellent walk-thru for connecting R to R-friendly flow package to FlowJo. In sum, this allows you to read gate info from FlowJo and analyze the contents in R. 

Continue reading "Fran's guide to flowing in R" »

June 11, 2009

I'm currently training FLAME skill to lvl1

June 03, 2009

known 7.5 issues

OK, now that the cat's out of the bag, here's what we already know isn't perfect. I'll update the list as issues are discovered and we'll also mark things fixed when 7.5.1 etc come out. 

- 7.2.5 worksapce's layouts may be missing charts, and iteration settings may be lost. 

- currenly, V8 .jo file support is limited to the workspace structures (gates, groups, etc.) only. 
- 3D platform will not work in any Virtual environment (VMware, parallels, etc.)
- accuri data linear parameters (DNA, Time parameter, ratio parameters etc.) may show strong picket fencing. 

- proxy support does not work properly. 

June 02, 2009

3 for 2 sale in effect

Looks like the savings are on - for the month of June, FlowJo licenses are three for price of two


Picture 1

7.5 is out


This week, the priority is to ensure a smooth transition from 7.2.5 to 7.5. We're doing a concurrent website rollover, to introduce the new branch of documentation for all new 7.5 features. If you encounter any problems during this transition, let us know - we're here to help :) 

FlowJo@treestar.com
800 366 6045

Dreamweaver-2

Graphic Credit - Don Gull

May 19, 2009

FlowJo binary mirrors

We've been experiencing some DNS and file permissions issues with our new mirror servers to distribute treestar's binary files, including the beta installer. Although the new beta version - 7.5 b 132, successor to 7.5cr2, has been posted - it remains either inaccessible or gives file permission error for some users. I'm looking into this.

We're looking for academic (.edu) mirrors for FlowJo. If you know anyone (who knows anyone) who maintains mirrors at your university, please let us know!

Specifically, we're looking for a place on the East coast US, Europe, South America, anywhere in Asia, and anywhere in Australia. 

May 14, 2009

attention Western New York

If you, or anyone you know, might use a copy of Alice Givans' Flow Cytometry book, Present Tense currently has it for $3, or "free" with a purchase of any other book. The book store is located in Batavia, NY at 101 Washington Ave. You may want to call ahead to see if it's still available, at five-eight-five,  815-7640